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Image Search Results
Journal: Plant Methods
Article Title: An optimised clearing protocol for the quantitative assessment of sub-epidermal ovule tissues within whole cereal pistils
doi: 10.1186/s13007-017-0217-z
Figure Lengend Snippet: Schematic representation of the steps involved in visualising the internal structures of ovules within cleared cereal pistils. a Plants were examined to identify tillers containing developing spikes. b , c Individual florets were removed from spikes to identify those at anthesis stage, achieved via observation of pollen on fingernails after anther squashing. c , d Whole pistils and anthers were gently removed and placed in fixative, followed by dehydration in an ethanol series and clearing in Hoyer’s solution. e Pistils were transferred to glass slides and covered with glass coverslips. f Samples were examined using DIC microscopy and Zeiss ZEN software
Article Snippet: Pistils were imaged using
Techniques: Microscopy, Software